Journal: Frontiers in Endocrinology

Article Title: Associations between seminal plasma osteopontin level and sperm motility in infertile men with asthenozoospermia

doi: 10.3389/fendo.2025.1487650

Figure Lengend Snippet: OPN concentration in the seminal plasma of infertile men with AZS (n=78) compared to the fertile men (n=80). (A) Scatter plots of OPN concentration in the seminal plasma of infertile men with AZS patients and fertile group using ELISA method. Data are presented as mean ± SEM; ∗∗∗∗ P < 0.0001. (B) ROC curves assessing the discriminative capability of OPN levels between infertile men with AZS and healthy fertile men, showing significant discriminative power with an AUC value of 0.793 (95% Confidence Interval, 0.724–0.863).

Article Snippet: According to the manufacturer’s instructions, seminal plasma OPN concentration was evaluated using the Human Osteopontin (OPN) Quantikine ELISA Kit (R&D systems, Minneapolis, MN, USA, catalog number DOST00).

Techniques: Concentration Assay, Clinical Proteomics, Enzyme-linked Immunosorbent Assay

Journal: Molecular Biomedicine

Article Title: Osteopontin promoted cardiac inflammation through increased interleukin-12 in acute myocarditis

doi: 10.1186/s43556-025-00333-z

Figure Lengend Snippet: OPN serves as a biomarker for myocarditis diagnosis. a Diagram illustrates detection of plasma OPN from acute myocarditis patients. b ELISA assay of IL-1-α, IL-1-β, IL-6 and TNF-α expression levels in plasma of acute myocarditis patients. p -value analyzed by Unpaired T-Test ( N = 6). c Echocardiographic parameters in acute myocarditis patients and Controls. LVEF: left ventricular ejection fraction. p -value analyzed by Unpaired T-Test ( N = 6). d ELISA assay of OPN levels in acute myocarditis patients. p -value analyzed by Unpaired T-Test ( N = 6). e Person analyses of correlation between OPN levels and LVEF, respectively ( N = 6). f ROC analyses determine specificity and sensitivity of OPN for acute myocarditis diagnosis ( N = 6)

Article Snippet: Plasma or cellular levels of OPN and cytokines, including IL-1β (Cat# EK0389), IL-1α (Cat# EK0392), IL-6 (Cat#EK0389), and TNF-α (Cat#EK0525), were measured using the OPN ELISA kit or corresponding cytokine ELISA kits (Boster, China) following the instruction.

Techniques: Biomarker Discovery, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing

Journal: British Journal of Cancer

Article Title: Plasma osteopontin as a biomarker of prostate cancer aggression: relationship to risk category and treatment response

doi: 10.1038/bjc.2012.345

Figure Lengend Snippet: Elevated plasma OPN is associated with CRPC-MET patients. ( A ) Baseline plasma OPN expression per risk category and in normal volunteers. Plasma OPN values were measured by ELISA assay in localised PCa patients (low, intermediate, high-risk and LR-locally recurrent cancer following radiotherapy); CRPC-MET – castrate-resistant metastatic PCa and non-cancer normal volunteers. (The mean value of plasma OPN values in CRPC-MET as previously published by Anborgh et al (2009) is shown by the black bar (219 μ g ml −1 )). CRPC-MET patients had significantly increased OPN values compared with localised PCas, including high-risk patients ( P -value 0.0001). ( B ) Receiver-operating curves (ROC) analysis for high-risk patients vs low- and intermediate-risk patients. For OPN: AUC=0.505; 95% CI=0.417–0.542; PSA: AUC=0.717, 95% CI=0.632–0.803; OPN+PSA: AUC=0.72, 95% CI=0.635–0.806. ( C ) Receiver-operating curves analysis for localised vs castrate resistance PCa patients with known metastatic disease. For OPN: AUC=0.935; 95% CI=0.889–0.982; PSA: AUC=0.943, 95% CI=0.872–1.0; OPN+PSA: AUC=0.969, 95% CI=0.918–1.0.

Article Snippet: The dual monoclonal human OPN ELISA kit (Catalog 900-142, Enzo Life Sciences, Plymouth Meeting, PA, USA), was used as previously published ( Anborgh et al , 2009 ) using the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay